Residue 25 in all the sequences:

Sequence
Residue 25
MACFA
D
HORSE
E
MACMU
D
FELCA
E
MACNE
E
CERTO
E
CARP
D


Residue frequency in position 25: 

Amino acid
Number
Frequency
A
0
0.0
C
0
0.0
D
3
42.9
E
4
57.1
F
0
0.0
G
0
0.0
H
0
0.0
I
0
0.0
K
0
0.0
L
0
0.0
M
0
0.0
N
0
0.0
P
0
0.0
Q
0
0.0
R
0
0.0
S
0
0.0
T
0
0.0
V
0
0.0
W
0
0.0
Y
0
0.0




The amino acids are coloured according to residue type:
Polar positive 
H K R
blue
Polar negative
 D E 
red
Polar neutral 
 S T N Q 
 green
Non-polar aliphatic 
 A V L I M 
 white
Non polar aromatic
 F Y W 
 purple
 P G 
 brown
C 
 yellow